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The application of pollen as bioindicator of the environmental state
Marečková, Kateřina ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
Pesticides and their excessive use lead to environmental pollution. Violation of the guidelines for their use disposal of empty containers could lead to contamination of water, soil and poisoning of animals and beneficial insects. Honey bee is useful creature on our planet. Good farming depends entirely on the pollination, but whole vegetal kingdom couldn’t exist it form known and used by mankind. Therefore, rules that protect these useful creatures against inadequate use of pesticides have been developed. This study focuses on the evaluation of the possibility to use bee products as bioindicators of the state of environment. Five active substances which are components of pesticides used in the treatment of agricultural field around Tasovice village were analysed in the pollen and honey. For sample preparation QuEChERS and SPE methods were used, gas chromatography with to mass spectrometric detection was employed as final analytical technique.
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Gel polymer electrolytes with fire retardands
Veselkova, Iuliia ; Jahn, Michal (referee) ; Sedlaříková, Marie (advisor)
This graduate work deals with the study and preparation of gel polymer electrolytes with flame retardants for lithium-ion batteries. The theoretical part describes the types of electrolytes, their features, benefits, how they differ and where they are used in detail. The basis of this section is gel electrolytes with flame retardants, to measure their electrical and electrochemical properties. The experimental part deals with the preparation of samples of gel electrolytes with different percentages of flame retardant, where varied species of flame retardants and measuring their electrical conductivity and potential windows. Impedance spectroscopy, cyclic voltammetry and dynamic-analytical thermal analysis were selected as measuring methods.
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Functional Ultrastructure of Hymenopteran Stingers: Devastating Spear or Delicate Syringe
ČERNÝ, Jan
In this study, we tested the hypothesis that a micro-serrated edge on the honey bee Apis mellifera stinger tip serves as a tool for more intensive crushing of cell membranes in the victim's tissues. This could have mechanical consequences as well as initiate metabolic pathways linked to cell membrane breakdown (e.g., production of biogenic amines). Accordingly, we found that hymenopteran species that use their stingers as an offensive or defensive weapon to do as much damage to the victim's body as possible had this cuticular microstructure. In parasitic hymenopterans, on the other hand, this structure was missing, as stingers are solely used to delicately transport venom to the victim's body in order to do little mechanical harm. We also demonstrated that the stinger lancets of the honey bee A. mellifera are living organs with sensilla innervated by sensory neurons and containing other essential tissues, rather than mere cuticular structures.
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Legal regulation of beekeeping
Kramperová, Barbora ; Fabšíková, Tereza (advisor) ; Damohorský, Milan (referee)
The diploma thesis discusses beekeeping and the basic rights and obligations of beekeepers and other persons responsible for ensuring the greatest possible protection of bees. The absence of a single comprehensive law results in the fragmentation of legislation into many private and public law regulations. The diploma thesis describes the main institutes of beekeeping law from its very birth to the present, with an evaluation of the pros and cons of individual development tendencies, together with an outline of the legal regulation, what de lege ferenda should be. This thesis is divided into three main parts, which are subsequently divided into chapters, subsections and possibly lower levels. The first part consists of four chapters dealing with the historical development of beekeeping and beekeeping law in our territory. The second part, focuses on private law areas of beekeeping, is divided into two chapters. The first chapter of these defines the bee in terms of private law. The second one deals with the issue of neighborhood rights, while the individual sub-chapters include the topics of neighborhood rights as a civil law concept, immigration, swarm law, liability for damage and its compensation, and methods of resolving neighborhood disputes. The last part deals with public law and is divided...
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Beekeeping and Its Use in Elementary School
Voska, Karel ; Andreska, Jan (advisor) ; Hanel, Lubomír (referee)
This thesis focuses on beekeeping and possibilities of its use in elementary schools. The first part is dedicated to the entire superfamily Apidae with a focus on the honeybee. However, other representatives are also mentioned across various social models, from solitary bees to those living in different levels of community life, up to large bee colonies. The second part of the thesis outlines the development of beekeeping, first at a general level and then within our country. It covers the inception of the relationship between humans and bees, the first pioneers of true beekeeping in the Mediterranean by Egyptians, Greeks, and Romans, and the development of beekeeping in our territory from the Middle Ages to the 20th century. The third and final part of the thesis is dedicated to the utilization of beekeeping in elementary schools. In this didactic section, various possibilities of incorporating bees into education across different subjects in elementary schools are described. The text includes proposals for topics and lesson plans, possible utilization of interdisciplinary relationships and tandem teaching, as well as suggestions for worksheets. The second part of the didactic work focuses on the beekeeping club at the elementary school, particularly its rules and types for its proper functioning....
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Technické a alternativní metody k šetrnému tlumení varoózy
NOVOTNÝ, Josef
Varroosis is a disease afflicting beehive on every continent except for Australia. Since 2000 the disease is known to be caused by the Varroa destructor mite. This thesis' first goal was to summarize information about this parasite and the methods limiting its impact. The second goal was to prepare and conduct a test verifying the effectiveness of the ultrasound method, which was carried out with the Varroa-Killer-Sound device. In order to enable the analysis, bee samples from two hives were chosen and those were then tested for the quantity of Varroa destructor females using the sugar shake method. The first sample group consisted of bees treated with the ultrasound method, the latter were control beehives, which were not experimentally treated. Furthermore, other factors were continually measured: temperature, relative humidity, and dew point of the external environment. The results of the research were analyzed by the STATISTICA 12 program.
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Sledování včel na vstupu včelího úlu
NOVOTNÝ, Petr
The main goal of this work is to design and put into operation a camera system for an intelligent beehive located at the Experimental and Educational Apiary of the Biological Center of the ASCR, v. VI in the area of the Dendrological Garden on Branišovská Street No. 31 in Č. Budějovice and to create software for monitoring, counting and space identification of bees at the entrance of a beehive.The system will consist of cameras connected to the Raspberry Pi where it will be processed using the image processing method. The obtained data will be processed on the server and stored in the database. Part of the obtained results will be displayed using Grafana's visualization tools.
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Infestace včelstev Nosema spp. v průběhu roku v různých lokalitách
DURČANSKÝ, Pavel
The nosem infection is a worldwide spread bee illness caused by two microsporidia (Nosema apis and Nosema ceranae). The aim of this thesis is to follow the occurrence of Nosema spp. and density of infection in the selected bee colonies considering the relation between N. apis, N. ceranae, climatic conditions and the condition of each bee colony concerned. In my thesis, I have used two ways of originator identification. The first was microscopy, using which we have discovered significant changes in the number of spores in one bee colony within one year. After the experiment had finished, we evaluated the number of measured spores in connection with the outside temperature, humidity, number of colonized frames, extenders, gentleness of bees and their sitting on the honey combs. The second way was using the PCR method. Through this method we have identified the percentage of positive bee colonies on the selected locations. Furthermore, we have confirmed the occurrence of each of the originators and evaluated if there is a mutual influence of Nosema spp. between bee colonies.
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Analýza spadu na podložky na dně včelího úlu
CHLUBNA, Marek
This bachelor's thesis is dealing with methods of computer vision and use of convolutional neural networks for the purpose of detection and counting the Varroa destructor on the pad removed from the bottom of the beehive. A research of such methods and convolutional neural networks is done together with an implementation. Experiments are performed and the results are made with the vision of using the results of this thesis for creating a mobile phone application.
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Analýza pohybu včelstva po plástu
HUMPÁL, Jan
This Bachelor's thesis considers the application of methods of computer vision and use of convolutional neural networks for purpose of detection and localization of the queen bee crawling amongst other bees on a honeycomb removed from a beehive. A research of such methods is done together with an attempted implementation, keeping in mind a possibility of using the results of this thesis for implementing a mobile phone application.
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